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Laboratory Operations: Phlebotomy & specimen handling – page 3

42 Laboratory Operations MCQs on Phlebotomy & specimen handling with answers and explanations.

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Q41HardPhlebotomy & specimen handling

A patient has a hematocrit of 60%. Using C = 1.85 × 10⁻³ × (100 − Hct) × V, how much 3.2% citrate is needed for 4.5 mL of blood?

Answer: B. 0.33 mL

C = 0.00185 × (100 − 60) × 4.5 = 0.00185 × 40 × 4.5 = 0.333 mL. The standard 0.5 mL would be too much citrate because plasma volume is reduced.

ID MG-LOP-0071 · Found a mistake? Report it
Q42HardPhlebotomy & specimen handling

Blood cultures are collected into aerobic and anaerobic bottles using a butterfly (winged) set. Which bottle should be filled first and why?

Answer: A. Aerobic, because air in the tubing enters the first bottle

The tubing contains air, which enters the first bottle filled. Filling the aerobic bottle first keeps oxygen out of the anaerobic bottle.

ID MG-LOP-0082 · Found a mistake? Report it
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