MLT GlobeBlood Banking: Blood components & storage – page 6
109 Blood Banking MCQs on Blood components & storage with answers and explanations.
Deglycerolized red cells prepared in a closed system and stored in AS-3 at 1–6 °C expire after:
A closed-system deglycerolization device allows 14 days of storage. Open-system deglycerolized cells expire in 24 hours.
Red cells collected in CPDA-1 without an additive solution must have a hematocrit no higher than:
Packed CPDA-1 red cells must have a hematocrit ≤ 80% so that enough glucose remains in the small plasma volume for 35 days.
Under AABB standards, a leukocyte-reduced whole-blood-derived platelet concentrate must contain fewer than:
Leukoreduced whole-blood-derived platelets must contain < 8.3 × 10^5 leukocytes per unit. The limit of < 5 × 10^6 applies to leukoreduced red cells and apheresis platelets.
After leukocyte reduction by filtration, a red cell unit must still contain at least what proportion of the original red cells?
AABB standards require that leukoreduced red cells retain at least 85% of the original red cell content. The 75% figure refers to the 24-hour in vivo recovery criterion for stored red cells.
A new red cell storage system is evaluated. What is the minimum mean 24-hour post-transfusion red cell recovery required for approval?
At least 75% of transfused stored red cells must remain in circulation 24 hours after transfusion. This criterion is used to set the shelf life of red cells in each anticoagulant or additive solution.
Red cells frozen by the low-glycerol (about 20%) method need rapid freezing and storage at:
The low-glycerol method gives less protection, so cells are frozen rapidly in liquid nitrogen and stored at −120 °C or colder (nitrogen vapour). High-glycerol (40%) units are stored at −65 °C or colder.
During deglycerolization of a frozen red cell unit, severe hemolysis occurs, and the unit clogs the washing set. The donor most likely has:
Hemoglobin S cells gel and lyse in the hypertonic solutions used for deglycerolization, so units from donors with sickle trait need a modified procedure. G6PD deficiency does not cause this effect.
Under AABB standards, a granulocyte concentrate collected by apheresis should contain at least:
At least 75% of granulocyte units must contain ≥ 1.0 × 10^10 granulocytes. The figures 3.0 × 10^11 and 5.5 × 10^10 are platelet content requirements.
During shipment, platelets may be held at room temperature without agitation for a maximum of:
Platelets may go up to 24 hours without agitation, as in transport. Longer periods without agitation cause pH to fall and reduce platelet quality.