MLT GlobeABO and Rh(D) blood grouping
Blood Bank · also called Blood group, Blood type, ABO typing, Rh typing
What it measures
Identifies the A and B antigens on red cells and the matching antibodies in plasma, and tests for the RhD antigen. Required before transfusion, in pregnancy and for donors.
Specimen and preparation
- Specimen: Whole blood (EDTA)
- Tube: Pink top
- Preparation: Label the sample at the bedside with two patient identifiers; many labs require a second sample to confirm group before issuing blood.
Reference range
| Group | Range |
|---|---|
| All | Reported as A, B, AB or O and RhD positive or negative |
Reference ranges differ between laboratories, methods and populations. Always use the range printed on your own lab report.
Method
Forward (cell) and reverse (serum) grouping by tube, slide, gel column or microplate agglutination; anti-D typing
Good to know
Forward and reverse results must agree; discrepancies occur in newborns, elderly patients, weak subgroups, cold antibodies and after stem cell transplant. Weak D may need extra testing.
Related tests
📱 Practise with a timer, track your score and earn certificates in the free MLT Globe app – Google Play or practise online.